Enhancement of the endogenous cytokinin concentration in poplar by transformation with Agrobacterium T-DNA gene ipt.
Identifieur interne : 004B40 ( Main/Exploration ); précédent : 004B39; suivant : 004B41Enhancement of the endogenous cytokinin concentration in poplar by transformation with Agrobacterium T-DNA gene ipt.
Auteurs : K. Von Schwartzenberg [France] ; P. Doumas ; L. Jouanin ; G. PilateSource :
- Tree physiology [ 1758-4469 ] ; 1994.
Abstract
The agrobacterial isopentenyltransferase (ipt) gene, under the control of its native promoter, was transferred to poplar (Populus tremula x P. alba) by an Agrobacterium co-cultivation method. The ipt-transformed stem explants developed calli that regenerated many buds in the absence of exogenous cytokinins. Microcuttings of the ipt transformants exhibited frequently branching shoots with short internodes that were unable to root. In this material, the concentrations of zeatin, zeatin riboside and isopentenyladenosine, determined by an indirect enzyme-linked immunosorbent assay (ELISA), were 4.8-. 17.1- and 14.6-fold higher, respectively, than in non-transformed shoots. Results are discussed with regard to cytokinin metabolism.
DOI: 10.1093/treephys/14.1.27
PubMed: 14967631
Affiliations:
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Pilate</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1994">1994</date><idno type="RBID">pubmed:14967631</idno><idno type="pmid">14967631</idno><idno type="doi">10.1093/treephys/14.1.27</idno><idno type="wicri:Area/Main/Corpus">004B54</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">004B54</idno><idno type="wicri:Area/Main/Curation">004B54</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">004B54</idno><idno type="wicri:Area/Main/Exploration">004B54</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Enhancement of the endogenous cytokinin concentration in poplar by transformation with Agrobacterium T-DNA gene ipt.</title><author><name sortKey="Von Schwartzenberg, K" sort="Von Schwartzenberg, K" uniqKey="Von Schwartzenberg K" first="K" last="Von Schwartzenberg">K. Von Schwartzenberg</name><affiliation wicri:level="3"><nlm:affiliation>Institut National de la Recherche Agronomique, Centre de Recherches Orléans, Station d'Amélioration des Arbres Forestiers, 45160 Ardon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut National de la Recherche Agronomique, Centre de Recherches Orléans, Station d'Amélioration des Arbres Forestiers, 45160 Ardon</wicri:regionArea><placeName><region type="region" nuts="2">Centre-Val de Loire</region><region type="old region" nuts="2">Région Centre</region><settlement type="city">Ardon</settlement></placeName></affiliation></author><author><name sortKey="Doumas, P" sort="Doumas, P" uniqKey="Doumas P" first="P" last="Doumas">P. Doumas</name></author><author><name sortKey="Jouanin, L" sort="Jouanin, L" uniqKey="Jouanin L" first="L" last="Jouanin">L. Jouanin</name></author><author><name sortKey="Pilate, G" sort="Pilate, G" uniqKey="Pilate G" first="G" last="Pilate">G. Pilate</name></author></analytic><series><title level="j">Tree physiology</title><idno type="eISSN">1758-4469</idno><imprint><date when="1994" type="published">1994</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The agrobacterial isopentenyltransferase (ipt) gene, under the control of its native promoter, was transferred to poplar (Populus tremula x P. alba) by an Agrobacterium co-cultivation method. The ipt-transformed stem explants developed calli that regenerated many buds in the absence of exogenous cytokinins. Microcuttings of the ipt transformants exhibited frequently branching shoots with short internodes that were unable to root. In this material, the concentrations of zeatin, zeatin riboside and isopentenyladenosine, determined by an indirect enzyme-linked immunosorbent assay (ELISA), were 4.8-. 17.1- and 14.6-fold higher, respectively, than in non-transformed shoots. Results are discussed with regard to cytokinin metabolism.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">14967631</PMID><DateRevised><Year>2019</Year><Month>11</Month><Day>20</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1758-4469</ISSN><JournalIssue CitedMedium="Internet"><Volume>14</Volume><Issue>1</Issue><PubDate><Year>1994</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Tree physiology</Title><ISOAbbreviation>Tree Physiol</ISOAbbreviation></Journal><ArticleTitle>Enhancement of the endogenous cytokinin concentration in poplar by transformation with Agrobacterium T-DNA gene ipt.</ArticleTitle><Pagination><MedlinePgn>27-35</MedlinePgn></Pagination><Abstract><AbstractText>The agrobacterial isopentenyltransferase (ipt) gene, under the control of its native promoter, was transferred to poplar (Populus tremula x P. alba) by an Agrobacterium co-cultivation method. The ipt-transformed stem explants developed calli that regenerated many buds in the absence of exogenous cytokinins. Microcuttings of the ipt transformants exhibited frequently branching shoots with short internodes that were unable to root. In this material, the concentrations of zeatin, zeatin riboside and isopentenyladenosine, determined by an indirect enzyme-linked immunosorbent assay (ELISA), were 4.8-. 17.1- and 14.6-fold higher, respectively, than in non-transformed shoots. Results are discussed with regard to cytokinin metabolism.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Von Schwartzenberg</LastName><ForeName>K</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Institut National de la Recherche Agronomique, Centre de Recherches Orléans, Station d'Amélioration des Arbres Forestiers, 45160 Ardon, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Doumas</LastName><ForeName>P</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Jouanin</LastName><ForeName>L</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Pilate</LastName><ForeName>G</ForeName><Initials>G</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Canada</Country><MedlineTA>Tree Physiol</MedlineTA><NlmUniqueID>100955338</NlmUniqueID><ISSNLinking>0829-318X</ISSNLinking></MedlineJournalInfo></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1994</Year><Month>1</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1994</Year><Month>1</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1994</Year><Month>1</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">14967631</ArticleId><ArticleId IdType="doi">10.1093/treephys/14.1.27</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li></country><region><li>Centre-Val de Loire</li><li>Région Centre</li></region><settlement><li>Ardon</li></settlement></list><tree><noCountry><name sortKey="Doumas, P" sort="Doumas, P" uniqKey="Doumas P" first="P" last="Doumas">P. Doumas</name><name sortKey="Jouanin, L" sort="Jouanin, L" uniqKey="Jouanin L" first="L" last="Jouanin">L. Jouanin</name><name sortKey="Pilate, G" sort="Pilate, G" uniqKey="Pilate G" first="G" last="Pilate">G. Pilate</name></noCountry><country name="France"><region name="Centre-Val de Loire"><name sortKey="Von Schwartzenberg, K" sort="Von Schwartzenberg, K" uniqKey="Von Schwartzenberg K" first="K" last="Von Schwartzenberg">K. Von Schwartzenberg</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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